Reconstruction of the cosmic microwave background lensing for Planck

Aims. We prepare real-life cosmic microwave background (CMB) lensing extraction with the forthcoming Planck satellite data by studying two systematic effects related to the foreground contamination: the impact of foreground residuals after a component separation on the lensed CMB map, and the impact of removing a large contaminated region of the sky. Methods. We first use the generalized morphological component analysis (GMCA) method to perform a component separation within a simplified framework, which allows a high statistics Monte-Carlo study. For the second systematic, we apply a realistic mask on the temperature maps and then restore them with a recently developed inpainting technique on the sphere. We investigate the reconstruction of the CMB lensing from the resultant maps using a quadratic estimator in the flat sky limit and on the full sphere. Results. We find that the foreground residuals from the GMCA method does not significantly alter the lensed signal, which is also true for the mask corrected with the inpainting method, even in the presence of point source residuals

Reconstruction of the CMB lensing for Planck

We prepare real-life Cosmic Microwave Background (CMB) lensing extraction with the forthcoming Planck satellite data, by studying two systematic effects related to the foregrounds contamination: the impact of foreground residuals after a component separation on the lensed CMB map, and of removing a large contaminated region of the sky. We first use the Generalized Morphological Component Analysis (GMCA) method to perform a component separation within a simplified framework which allows a high statistics Monte-Carlo study. For the second systematic, we apply a realistic mask on the temperature maps and then, restore them using a recent inpainting technique on the sphere. We investigate the reconstruction of the CMB lensing from the resultant maps using a quadratic estimator in the flat sky limit and on the full sphere. We find that the foreground residuals from the GMCA method does not alter significantly the lensed signal, nor does the mask corrected with the inpainting method, even in the presence of point sources residuals.Comment: 14 pages, 7 figures, major update to account for the impact of the point sources emissio

A hybrid approach to CMB lensing reconstruction on all-sky intensity maps

Based on realistic simulations, we propose an hybrid method to reconstruct the lensing potential power spectrum, directly on PLANCK-like CMB frequency maps. It implies using a large galactic mask and dealing with a strong inhomogeneous noise. For l < 100, we show that a full-sky inpainting method, already described in a previous work, still allows a minimal variance reconstruction, with a bias that must be accounted for by a Monte-Carlo method, but that does not couple to the deflection field. For l>100 we develop a method based on tiling the cut-sky with local 10x10 degrees overlapping tangent planes (referred to in the following as "patches"). It requires to solve various issues concerning their size/position, non-periodic boundaries and irregularly sampled data after the sphere-to-plane projection. We show how the leading noise term of the quadratic lensing estimator applied onto an apodized patch can still be taken directly from the data. To not loose spatial accuracy, we developed a tool that allows the fast determination of the complex Fourier series coefficients from a bi-dimensional irregularly sampled dataset, without performing an interpolation. We show that the multi-patch approach allows the lensing power spectrum reconstruction with a very small bias, thanks to avoiding the galactic mask and lowering the noise inhomogeneities, while still having almost a minimal variance. The data quality can be assessed at each stage and simple bi-dimensional spectra build, which allows the control of local systematic errors.Comment: A&A version. Mostly english correction

Separation of anomalous and synchrotron emissions using WMAP polarization data

The main goals of this study is to use the information from both WMAP intensity and polarization data to do a separation of the Galactic components, with a focus on the synchrotron and anomalous emissions. Our analysis is made at 23 GHz where the signal-to-noise ratio is the highest and the estimate of the CMB map is less critical. Our estimate of the synchrotron intensity is based on an extrapolation of the Haslam 408 MHz data with a spatially varying spectral index constrained by the WMAP 23 GHz polarization data and a bi-symmetrical spiral model of the galactic magnetic field with a turbulent part following a -5/3 power law spectrum. The 23 GHz polarization data are found to be compatible with a magnetic field with a pitch angle p=-8.5 degrees and an amplitude of the turbulent part of the magnetic field 0.57 times the local value of the field, in agreement with what is found using rotation measures of pulsars and polarized extinction by dust. The synchrotron spectral index between 408 MHz and 23 GHz obtained from polarization data and our model of the magnetic field has a mean value of Beta=-3.00 with a limited spatial variation with a standard deviation of 0.06. When thermal dust, free-free and synchrotron are removed from the WMAP intensity data, the residual anomalous emission is highly correlated with thermal dust emission with a spectrum in agreement with spinning dust models. Considering a classical model of the large scale Galactic magnetic field, we show that the polarization data of WMAP are in favor of a soft synchrotron intensity highly correlated with the 408 MHz data. Furthermore the combination of the WMAP polarization and intensity data brings strong evidence for the presence of unpolarized spinning dust emission in the 20-60 GHz range.Comment: accepted by A and

A Regulatory Mechanism Involving TBP-1/Tat-Binding Protein 1 and Akt/PKB in the Control of Cell Proliferation

TBP-1 /Tat-Binding Protein 1 (also named Rpt-5, S6a or PSMC3) is a multifunctional protein, originally identified as a regulator of HIV-1-Tat mediated transcription. It is an AAA-ATPase component of the 19S regulative subunit of the proteasome and, as other members of this protein family, fulfils different cellular functions including proteolysis and transcriptional regulation. We and others reported that over expression of TBP-1 diminishes cell proliferation in different cellular contexts with mechanisms yet to be defined. Accordingly, we demonstrated that TBP-1 binds to and stabilizes the p14ARF oncosuppressor increasing its anti-oncogenic functions. However, TBP-1 restrains cell proliferation also in the absence of ARF, raising the question of what are the molecular pathways involved. Herein we demonstrate that stable knock-down of TBP-1 in human immortalized fibroblasts increases cell proliferation, migration and resistance to apoptosis induced by serum deprivation. We observe that TBP-1 silencing causes activation of the Akt/PKB kinase and that in turn TBP-1, itself, is a downstream target of Akt/PKB. Moreover, MDM2, a known Akt target, plays a major role in this regulation. Altogether, our data suggest the existence of a negative feedback loop involving Akt/PKB that might act as a sensor to modulate TBP-1 levels in proliferating cells

SPR imaging biosensor for the 20S proteasome: sensor development and application to measurement of proteasomes in human blood plasma

The 20S proteasome is a multicatalytic enzyme complex responsible for intracellular protein degradation in mammalian cells. Its antigen level or enzymatic activity in blood plasma are potentially useful markers for various malignant and nonmalignant diseases. We have developed a method for highly selective determination of the 20S proteasome using a Surface Plasmon Resonance Imaging (SPRI) technique. It is based on the highly selective interaction between the proteasome’s catalytic β5 subunit and immobilized inhibitors (the synthetic peptide PSI and epoxomicin). Inhibitor concentration and pH were optimized. Analytical responses, linear ranges, accuracy, precision and interferences were investigated. Biosensors based on either PSI and epoxomicin were found to be suitable for quantitative determination of the proteasome, with a precision of ±10% for each, and recoveries of 102% and 113%, respectively, and with little interference by albumin, trypsin, chymotrypsin, cathepsin B and papain. The proteasome also was determined in plasma of healthy subjects and of patients suffering from acute leukemia. Both biosensors gave comparable results (2860 ng·mL-1 on average for control, and 42300 ng·mL-1 on average for leukemia patients)

Regulation of the High Affinity IgE Receptor (FcεRI) in Human Neutrophils: Role of Seasonal Allergen Exposure and Th-2 Cytokines

The high affinity IgE receptor, FcεRI, plays a key role in the immunological pathways involved in allergic asthma. Previously we have demonstrated that human neutrophils isolated from allergic asthmatics express a functional FcεRI, and therefore it was of importance to examine the factors regulating its expression. In this study, we found that neutrophils from allergic asthmatics showed increased expression of FcεRI-α chain surface protein, total protein and mRNA compared with those from allergic non asthmatics and healthy donors (p<0.001). Interestingly, in neutrophils isolated from allergic asthmatics, FcεRI-α chain surface protein and mRNA expression were significantly greater during the pollen season than outside the pollen season (n = 9, P = 0.001), an effect which was not observed either in the allergic non asthmatic group or the healthy donors (p>0.05). Allergen exposure did not affect other surface markers of neutrophils such as CD16/FcγRIII or IL-17R. In contrast to stimulation with IgE, neutrophils incubated with TH2 cytokines IL-9, GM-CSF, and IL-4, showed enhanced FcεRI-α chain surface expression. In conclusion, these results suggest that enhanced FcεRI expression in human neutrophils from allergic asthmatics during the pollen season can make them more susceptible to the biological effects of IgE, providing a possible new mechanism by which neutrophils contribute to allergic asthma

Planck 2013 results. XVII. Gravitational lensing by large-scale structure

On the arcminute angular scales probed by Planck, the cosmic microwave background (CMB) anisotropies are gently perturbed by gravitational lensing. Here we present a detailed study of this effect, detecting lensing independently in the 100, 143, and 217 GHz frequency bands with an overall significance of greater than 25σ. We use thetemperature-gradient correlations induced by lensing to reconstruct a (noisy) map of the CMB lensing potential, which provides an integrated measure of the mass distribution back to the CMB last-scattering surface. Our lensing potential map is significantly correlated with other tracers of mass, a fact which we demonstrate using several representative tracers of large-scale structure. We estimate the power spectrum of the lensing potential, finding generally good agreement with expectations from the best-fitting ΛCDM model for the Planck temperature power spectrum, showing that this measurement at z = 1100 correctly predicts the properties of the lower-redshift, later-time structures which source the lensing potential. When combined with the temperature power spectrum, our measurement provides degeneracy-breaking power for parameter constraints; it improves CMB-alone constraints on curvature by a factor of two and also partly breaks the degeneracy between the amplitude of the primordial perturbation power spectrum and the optical depth to reionization, allowing a measurement of the optical depth to reionization which is independent of large-scale polarization data. Discarding scale information, our measurement corresponds to a 4% constraint on the amplitude of the lensing potential power spectrum, or a 2% constraint on the root-mean-squared amplitude of matter fluctuations at z ~ 2

Planck 2013 results. I. Overview of products and scientific results

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